ABSTRACT
The aim of this study was to determine the effect of plant spices (Thymus vulgaris, Murraya koenigii, Ocimum gratissimum and Piper guineense) on hemoglobin glycation (HbA1c), lactate dehydrogenase (LDH), glucose-6-phosphate dehydrogenase (G6PD) and red blood cell (RBC) indices in alloxan- induced diabetic rats. Study Design: The animals were grouped into six of 5 rats each. Groups II, III, IV, V and VI were induced diabetes by intraperitoneal injection of alloxan monohydrate with a dose of 170mg/kg body weight. Group I was the control, group II diabetic control and group III to VI were the experimental group. Crude aqueous extracts (500mg/kg body weight) of the spices were orally administered to the rats. Methodology: Blood samples were collected by cardiac puncture after fasting overnight and standard methods were used for the extraction of spices and, determination of biochemical and hematological parameters. Place and Duration of Study: The study was carried out at Enugu State University Teaching Hospital from September 2012 to January 2013. Result: The result showed that glycated hemoglobin, fasting blood sugar and lactate dehydrogenase decreased significantly (P<0.05) while, glucose-6-phosphate dehydrogenase increased significantly (P<0.05) compared to diabetic control group but, no significant difference (P<0.05) was observed compared to normal control group. There were no significant differences (P<0.05) in red blood cell indices compared to diabetic control and normal control. Conclusion: This study suggested that the spices extracts can be used to control diabetes and prevent its complications on antioxidant enzymes.
ABSTRACT
Biochemical Changes in serum of male albino rats treated with aqueous leaf extract of Phyllanthus annus were examined. 50mg/kg and 100mg/kg of the extracts were administered orally and once daily to group I and group II of test animals respectively for 28 days. The third group which served as control received distilled water only. On the 29th day, the rats which had been fasted overnight were dissected under chloroform anaesthesia and blood was collected directly from their heart. The blood was allowed to clot and centrifuged to obtain serum which was kept in a refrigerator at - 200C until its usage for the analysis of the following parameters; alanine and aspartate transaminases, alkaline and acid phosphatases, haemoglobin concentration, high and low density lipoproteins. The result indicates significant (P<0.05) increases in AST, ALT and ACP levels in tests groups compared to the control animals. There were non-significant elevation in the concentration of ALP, HDL, LDL and haemoglobin. These results suggest that P amarus may be toxic if abused but if properly administered can be use for its medicinal values.